Effects of subconjunctival injected AMD 3100,a specific antagonist of CXCR4,on alkali-induced corneal neovascularization in mice

Objective To investigate the anti-angiogesis effects of subconjunctival injectedAMD 3100 on alkali-induced corneal neovascularization in mice. Methods Alkali burn was applied to induce the formation of cornea neovasculafization(CNV) in mice.On the day after alkali bum,AMD 3100 5 μg(10 μL) and normal saline 10 μl were respectively injected into subconjunctival tissue of mice,one time every other day,seven times in total.Corneal inflammation index was observed under slit lamp at different time points.The numbers 0of inflammatory cells were detected by histopathological examination,and microvessel density(MVD) in the cornea was observed by immunohistochemistry. Results At different time points after alkali burn,corneal inflammation index,inflammatory cells and MVD of experimental group were significantly less than of the control group(P0.05). Conclusion AMD3100 can effectively inhibit the growth of CNV,which may be a result of the decrease of inflammatory response after alkali bum.