Production of Specific Proteins during Graft Compatibility/Incompatibility Response of Heterograft Combination of Cucurbitaceae

The production of specific proteins during graft compatible/incompatible heterograft combination of cucurbitaceae was studied by SDS-PAGE electrophoresis.The seedlings of watermelon(Citrullus lanatus(Thunb.)) cultivar 8424 and melon(Cucumis melo L.) cultivar Yongtian No.3 were grafted onto the compatible rootstock of winter squash(Cucurbita maxima duch.ex lam) A(TLZ-B2-1-24) and incompatible rootstock winter squash B(Z079-1-2-17),to format the compatibility and incompatibility response of heterograft combination of watermelon/ winter squash A and melon/winter squash A,and watermelon/winter squash B and melon/winter squash B,respectively.The results showed as the follows:a decrease in a 39 kDa callus protein band at the winter squash A stock of the compatible union was observed during the 10 days after grafting,and the culls proteins of scion(watermelon and melon) were still kept.During the incompatible heterograft stage,two specific proteins(36 kDa for watermelon and 28 kDa for melon) were expressed in the scion,and the original protein of winter squash B were declined seriously and a 38 kDa callus protein were decreased.Those results presented here demonstrated the identification of the proteins related to graft compatibility/incompatibility response.These findings allow the assumption that molecular change mechanism takes place during graft formation in compatible/incompatible combinations for the first time.