Screening Phage Displayed 12-peptide Library for Specific Ouabain Conjugated Peptides

Objective Endogenous ouabain (EO) has been hypothesized to play a certain role in the pathogenes of hypertension. The purpose of the study was to find ouabain conjugated peptides(OCP) blocking the endogenous ouabain(EO) action on sodium pump, Methods Screening the phage displayed 12 peptide library by biopaning for OCP. The sequences of each selected peptide was determined and the sequence was analyzed through internet. Results Three peptides were selected. Peptide A(12 peptide) was occupied in 66.7%(8/12),Peptide B(8 peptide) 16.7%(2/12)and peptide C (12 peptide) 8.3%(1/12). There was only one case without insertron. Protein of the analysis showed that there were no homogeneous between peptide A, B, C and sodium pump. Conclusion Investigation of the sequence of OCP provides an important experimental foundation for ouabain research.