The Experiment Study on Composite Denshen Pilulae(CDP) in Prevention of Restenosis in Rabbits

Objective To investigate the mechanism of CDP in prevention and treatment of restenosis in rabbits.Methods 30 white male rabbits were randomly divided into 3 groups: control group(n=10);model group(whose intima was destroyed by balloon and was given hypercholesteroic diet,n=10) and treatment group(whose intima was destroyed by ballon and was given hypercholesteroic diet plus CDP 150mg/d,n=10). After 8 weeks,rabbits were killed and the local iliac arteries were sectioned for analysis of pathological morphology and immunohistochemistry analysis[the expression of metalloproteinases(MMPs) MMP2,MMP9].Angiogram of iliac artery was performed in all rabbits.Results(1)According to the angiogram of iliac artery,the stenosis of artery lumen diameter decreased 27.8% in treatment group compared with model group.According to automatic pathological morphology analysis,the thickness of intima decreased 41% and intima area decreased 52% in the treatment group compared with the model group.The intima thickness/medium thickness of arteries ratio decreased 22%,the area ratio decreased 38% in treatment group compared with the model group(P0.01).(2)According to the immunohis to chemistry analysis,the expressions of MMP2、MMP9 were 11.3±3.65,17.2±1.88 in control group,23.4±4.61,28.2±2.38 in model group and 16.2±3.03,20.1±4.01 in treatment group.The expressions of MMP2 and MMP9 were higher in model group than in control group.And the expressions of MMP2 and MMP9 were significantly lower in treatment group than in model group(P0.01).(3)The thickening of vascular neointima and stenosis degree of vascular lumen were correlated well with MMP2、MMP9(r=0.896,P0.01). Conclusions MMP and TIMP(metalloproteinase inhibitors) play very important role in the restenosis process of artery.CDP can inhibit the thickness of vascular neointima after balloon injury.