On immunolocalization of Ig-like proteins in shrimp Litopenaeus vannamei

In the paper, an attempt was made to analysis the immunolocalization of Ig-like proteins that bound to anti-human Ig (immunoglobulin, Ig) in shrimp L. vannamei using modern methods of Western-blotting and immunohistochemistry. The results showed two types of protein which reacted strongly with goat an-ti-humanIg in the shrimp hemolymph, whose molecular weights were about 70 ku and 62 ku respectively. And the outer membrane of the hyalocyte and five tissues including lymphatic-like organ, hepatopancreas, heart, intestine and stomach were also bound with anti-human Ig specially, of which, the lymphatic-like organ and hepatopancreas were stained stronger than other samples. These results indicated that there do exist Ig-like proteins in the shrimp, which localized not only in hemolymph but also in haemocytes, lymphatic-like organ and hepatopancreas.