2012•Zhongguo xiandai yixue/Zhongguo xiandai yixue zazhiRequires access
Palmitate induces apoptosis in rat aortic endothelial cells
Guoliang Liu
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Abstract
【Objective】We investigated the effect of ghrelin on palmitate-induced apoptosis in rat aortic endothelial cells.【Methods】Rat aortic endothelial cells were incubated in 0.5% BSA or 0.5% BSA complexed to palmitate(0.1 mm,0.2 mm,0.3 mm).Cell viability was assessed by MTT assay.Apoptosis was detected using an annexin V/PI double staining assay.Spectrofluorometer assay caspase-3 activity.【Results】Palmitate decreased cell viability compared with control group as assessed by MTT asssy.Palmitate increased cell apoptosis compared with BSA group as assessed by an annexin V/PI double staining assay(P 0.05 or P 0.01).Palmitate increased caspase-3 activity compared with BSA group(P 0.05 or P 0.01).【Conclusions】These findings provide the evidence that palmitate could induce apoptosis in rat aortic endothelial cells.High level of free fatty acid could injury endothelial cells directly.