The effects of cobravenom cytotoxin IVon morphine-induced behavioral sensitization in mice

Objective To investigate the effects of cobravenom cytotoxin Ⅳon morphine-induced sensitization in mice.Methods Locomotor activity was measured every 5 minutes for 1 hour immediately after administration with cobravenom cytotoxin Ⅳin mice.The morphine-induced sensitization models were established in mice by subcutaneous injection with morphine 10mg/kg,once a day,for 7 days,and the mice were treated with the combination of morphine(sc) with cytotoxin Ⅳ(ip) to observe the effects of cytotoxin Ⅳon the behavioral sensitization to morphine in mice.Results After treated with different doses of cytotoxin Ⅳ (0.027,0.04,0.08mg/kg,respectively),the locomotor activity of the mice was reduced,hyperactivity was inhibited,and the expression of morphine-induced behavioral sensitization was blocked,in which the effects of middle and high-dose of cytotoxin Ⅳ were more significant.Conclusion The cobravenom cytotoxin Ⅳ has inhibitory effects on central nervous system and can prohibit the formation of morphine addictive behavior,which suggests that cobravenom cytotoxin Ⅳ may have intervention effect on psychological dependence to morphine.