The Study on regulation of melanogenesis in the responese of murine melanoma cells to Radix Scutellariae

To study the regulation of proliferation, melanogenesis and tyrosinase activity in the responese of B16F murine melanoma cells to Radix Scutellariae. B16F murine melanoma cell was cultured in vitro,MTT was used to study the proliferation of B16F murine cells. The melamin was mesured by NaOH-assay, and the tyrosinase activity was detected by DOPA-oxidation. in the concentrations ranging from 0.01 to 1.0 g·L-1, Radix Scutellariae could significantly promote the proliferation, melanin content and tyrosinase activity, and the most potent effects were achieved at the concentration of 1 g·L-1.