Construction of Monascus full-length cDNA library with SMART approach

Objective Construction of Monascus cDNA Library provide foundations for the research of Monascus functional genes and screening,cloning genes related to the synthesis pathway of secondary metabolites in Monascus.Methods A cDNA library was constructed based on SMART system.The number of the clones and the recombination rate of the library were determined by plate coating and restrictive digestion,and the size of inserted fragment was determined by PCR.Results The primary library capacity was 2.03×105,average inserted fragment size was about 1 000 bp and the percentage of recombination were 94%.Conclusion The library met the requirement for cloning target genes and expressing target proteins.