Determination of Ascorbic Acid by Enzymatic Catalytic Spectrophotometry

The spectrophotometry for the determination of ascorbic acid based on the inhibition of hemoglobin for the oxidation of H2O2 with acid chrome blue K was developed.The percentage inhibition(I%)of system is under the optimal experimental conditions.The calibration curve is linear in the range of 2.0×10-7—5.9×10-5mol/L with the detection limit of 7.0×10-8mol/L.The relative standard derivation is 2.5% at ascorbic acid concentration of 6.0×10-6mol/L(n=11).This method can be used for the determination of ascorbic acid in pharmaceuticals and vegetables with satisfactory results.