Effect of NF-κB on autophagy formation of human hepatoma cell

Objective To research the effect of NF-κB on autophagy formation of Human Hepatoma Cell HepG2.Methods The human hepatoma cell HepG2 was cultured in vitro and divided into SN50 group and control group,MTT assay was used to test the cell growth dynamically.Western blot analysis was performed to assess the expression of LC3-Ⅱ.The autophagy was observed using fluorescent microscope by monodansylcadaverin(MDC) staining.Results The inhibition ratio of HepG2 cells were(20.45±3.70)%,(31.94±4.20)% and(36.44±4.60)% after treatment of SN50 for 24,48 and 72 h,the difference was statistically significant than before(P 0.05).The expression of LC3-II had upregulation and fluorescent staining revealed the activity of autophagy.Conclusion Blocking of NF-κB can significantly inhibit the proliferation of HepG2 cells in a certain period of time,and the effect may associated with increased cell autophagy.