Impact of small interference RNA - induced silencing of adrenomedullin gene on the cell cycle in malignant melanoma cells

Objective To assess the impact of small interference RNA(siRNA)-induced silencing of Adrenomedullin(ADM) gene on the cell cycle in a malignant melanoma cell line A375.Methods Real-time quantitative reverse transcription PCR(qRT-PCR) was performed to select the most efficient siRNA for the experiment.A375 cells were classified into 3 groups to be transfected with the selected siRNA(test group),non-specific sequences(negative control group),or remain untransfected(blank control group).Cell cycle was detected by flow cytometry after knocked out ADM by siRNA at 48 hours.Results Flow cytometry revealed that the proportion of cells in G2 / M phase were dramatically increased in siRNA-transfected cells(11.360 ± 1.224) % than that of remaining untransfected group cells(1.020 ± 0.990) % and non-specific sequences group cells(4.150 ± 1.032) %(P 0.05);No obvious differences were detected on the percentages of cells in G1 / G0 and S phase between both blank control group(68.567 ± 3.653,30.417 ± 4.582) % and negative control group(70.530 ± 3.008,23.313 ± 4.645) % with test group(69.443 ± 2.023,19.197 ± 0.890) %(both P 0.05).Conclusion ADM siRNA can inhibit the proliferation of siRNA cells via G2 / M pathway.