Expression of mGluR7 in the Subventricular Zone in Adult Rats Following Focal Cerebral Ischemia-reperfusion

Objective Through investigating the expression of metabotropic glutamate receptor 7 subtype(mGluR7)in the subventricular zone in adult rats following focal cerebral ischemia-reperfusion,to explore its role in neurogenesis.Methods The model of acute reperfusion injury after cerebral ischemia in rat was made by middle cerebral artery occlusion(MCAO).All animals were allowed 2 h ischemia and sacrificed after different durations of reperfusion(6 h,24 h,72 h,7 d,14 d,28 d).The expression of mGluR7 was detected with an immunohistochemical approach.Results Compared with the control group,the number of mGluR7 immunoreactive cells in the subventricular zone at 24 h after reperfusion in the ischemic side started to increase significantly and peaked at 3 d.However,at 28 d after reperfusion,the number of mGluR7 labeled cells decreased to basal level.In addition,the mGluR7 labeled cells were also detected in dorsal caudate putamen,lateral septal necleus,piriform endo-nucleus and some areas of cerebral cortex.Conclusion The study implies cerebral ischemia could up-regulate the expression of mGluR7 and induce the proliferation of nervous stem cells in the subventricle zone following ischemia.