Screening insertion-deletion mutation by DNA sequencing

DNA sequencing technology has played important roles in modern biological research.Although the second-generation high-throughput sequencing technology has been widely used in various disciplines,but conventional sequencing technology,such as the Sanger dideoxy termination sequencing technology remains irreplaceable.In this study,PCR products were amplified by the polymerase chain reaction(PCR) and 20 genomic DNA of cynomolgus monkey,then PCR products were purified and sequenced.The peaks of PCR products sequencing were analyzed,including weak signal or no signal,poly structure,base insertion/deletion and so on.One method of screening insertion/deletion mutations based on the PCR products sequencing were proposed,which would improve the analysis validity and accuracy of the sequencing results.