2011•Chinese Journal of Clinical Rational Drug UseRequires access
Determination of rhein,emodin and chrysophanol in Chinese Medicine Tuihuang outside lotion by HPLC
Yan Dong-li
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Abstract
Objective To establish a method for determination of rhein,emodin and chrysophanol in Chinese Medicine Tuihuang outside lotion.Methods Determinedf rhein,emodin,chrysophanol by HPLC,column:Phenomenex Gemini 5μm C18 110A 4.6×250.0mm 5micron;Mobile phase:Methanol-0.1% phosphoric acid(85:15),flow rate:1.0ml/min,detection wavelength:254nm,column temperature:35℃.Results Rhein at 1.6576～33.1520μg/ml,emodin at 1.4976～29.9520μg/ml,chrysophanol at 1.7040～34.0800μg/ml range,had a good linear relationship(r=1).The average recovery rhein was 99.07%,RSD=0.85%(n=5);Emodin was 99.14%,RSD was 1.08%(n=5);Chrysophanol was 99.94%,RSD was 0.77%(n=5);The negative sample without interference.Conclusion This method is simple,stable,specific,and can be used for the quality control methods.