Nutritional effect of alanlyglutamine on traumatized rat

Purpose Trauma results in hypercatabolism and increasing the requirement for glutamine (Gln). It is necessary to supplement it. Due to its unstable and decomoposing to toxic pyrogluta,ic acid and ammonia, Gln is limited to use for parenteral nutrition, this problem of instability can be obviated by use of alanlyglutamine (Ala-Gln). Methods In this study, effects of Ala-Gln or Gln for intravenous nutrition on traimatized rats were investigated. A-ter injured, thirty-six male Wistatr rats weighting 200±10g were randomized to three groups separetely receiving standard amino acids solutions (AAS),AAS with Ala-Gln supplementation or AAS with Gln suppIementation through central vena for fourteen days,200mg nltrogen per day. dietary intake was weighed everyday and body weight every other day. On days 4 and 8, nitrogen balance was determined, on days 14, plasma proteins and Gln, peripheral lymphocyte blastogenesis and the content of DNA, RNA and protein in intestinal mucosa were measured. Results There was no significant difference in dietare intake and body weight among three groups. The nitrogen balance of Ala-Gln and Gln group on days 8 was remarkably improved compared with control group (P 0. 05). No evident difference was observed in plasma total protein, albumin and prealbumin. Fibronectin increased compared with preoperation (P 0. 05), which resulted from the wound and the stimulation of central venous catheter, but serum fibronectin was recovered better in Ala-Gln and Gln group than contro group (P 0. 05). Compared with control group,Ala-Gln and Gln group had higher concentration of plasma Gln, better T lymphocyte blastogenesis, higher content of DNA,RNA and protein in the small bewel mucosa. Conclution This indicates that Ala-Gln was decomposed Gln and alanine, both Ala-Gln and Gln can optimize protein metaboism, enhance immune function and promte the growth of intestinal mucosa, and the nutritional effects of Ala-Gln or Gln are the same.