Research on the apoptosis induced by chemotherapy under specific condition in colon carcinoma

Objective:To observe the apoptosis-inducing effect of 5-FU and oxaliplatin under specific condition in colon cancer cell line HT-29.Methods:HT-29 cells were separately treated with 80μg/ml 5-FU and 25μg/ml oxaliplatin for 8 hours.And the cellular apoptosis was analyzed by AnnexinV-FITC/PI double staining flow cytometry.Results:HT-29 cell line was detected by AnnexinV-FITC/PI double staining flow cytometry.Compared with the blank group,the proportion of apoptosis cells of the group treated with 80μg/ml 5-FU for 8 hours increased approximately 9.2%(P0.01).and the proportion of apoptosis cells of the group treated with 25μg/ml oxaliplatin for 8 hours increased approximately 21.1%(P0.01).Compared with the group treated with 80μg/ml 5-FU for 8 hours,the proportion of apoptosis cells of the group treated with 25μg/ml oxaliplatin for 8 hours increased approximately 11.9%(P0.01).Conclusion:5-FU and oxaliplatin under specific condition both can induce apoptosis of HT-29 cell line.Oxaliplatin resultes in stronger effect of apoptosis inducing than 5-FU in colon cancer cells.