2004•Sichuan dongwuRequires access
Optimal time to collect fertilized egg with clear pronuclei for making transgenic mouse
Xueling He, Hailin Yin
Open publisher page 0 citations
Abstract
Microinjection is the most efficient method to produce transgenic animal today. To obtain the fertilized eggs with clear male pronuclei is the key of success . This article figures out the optimal duration in obtaining the suitably fertilized eggs with clear male pronuclei of mouse after HCG(human chorionic gonadotropin) injection. The result showed that the optimal duration of obtaining the suitably fertilized eggs with clear male pronuclei of mouse was 25-27h after HCG injection.