ANTISENSE RNA OF SURVIVIN SENSITIVE DOXORUBICIN TO PANCREATIC CANCER CELL LINE PANC-1

Objective: To explore the effects of survivin antisense RNA on doxorubicin-induced apoptosis in pancreatic cancer cell line PANC-1.Methods: A survivin antisense eukaryotic vector pcDNA3-SVVas prepared in previous study was delivered into PANC-1 mediated by electroperforation.Cell survival fraction was determined using the trypan blue dye exclusion assay.Cell proliferation and MTT assay were tested to investigate the sensibility of transfected cells to doxorubicin.Apoptosis was detected by DNA gel electrophoresis.Results: We obtained two positive cell clone PANC-1/SVVas and PANC-1/neo by eletroporation.Compared to PANC-1 and PANC-1/neo cells,PANC-1/SVVas cells growth was significantly reduced(\$P\$0.05).By MTT assay,the IC_(50) of to PANC-1/SVVas,PANC-1/neo and PANC-1 cells were 0.285±0.012 μmol/L,1.528±0.317 μmol/L and 1.540±0.253 μmol/L respectively,the difference was significant by statistic analysis(P0.01).Agarose gel electrophoresis of genomic DNA from PANC-1/SVVas showed typical DNA ladder,but DNA from PANC-1/neo and PANC-1 did not.Conclusion: Survivin antisense RNA could enhance doxorubicin-induced apoptosis in pancreatic cancer cell line PANC-1.This may lay an experimental foundation for further research of gene therapy in pancreatic cancer.