Single Oocyst Separation of Eimeria maxima and Oocyst Amplification by Chicken

Coccidia oocysts existed in the small intestine in a clinical died chicken were collected using saturation saline floatation method.After sporulation of oocysts,single oocyst was separated by 2% agarose.Seven 5-day-old chickens were inoculated with the single oocyst.After two times of passage,Eimeria maxima oocysts identified by morphology were again infected to ten 10-day-old chickens,with 1.0×104 oocysts per bird.Thus,numbers of pure oocysts were obtained.The present result suggested that the single oocyst separating technique that used a blade cutting agar plate was a simple and convenient method,which had a higher success rate of single oocyst infection.Therefore,it is useful to amplify the pure oocysts.