The improvement of bacteria sample preparation method for scanning electron microscope observation

The conventional SEM sample preparation needs multiple steps including fixing,rinsing,and dehydrating.Because the volume of microbiology sample or unicellular sample is so small,so each step of preparation needs centrifugal collection.However,multiple centrifuging may cause loss of the sample.In this study,filter paper pack is used to collect sample.This method significantly improves the efficacy of the sample preparation and enhances the observing effect of the sample.