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Antagonistic Effects of Limulus Anti-Lipopolysaccharide Factor on High Mobility Group Box-1 in vitro

Donghui Su

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Abstract

Objective To investigate the effects of limulus anti-lipopolysaccharide factor(LALF) on the late proinflammatory cytokine of high mobility group box 1(HMGB1).Methods The primary mouse macrophages were cultured and stimulated by HMGB1. Macrophage migration was determined by the use of chemotaxis chamber.ELISA quantitated the culture supernatants for TNF-α production.LALF was added to explore the effects of HMGBI and TNF-α production in culture.Results The chemotactic index of macrophages and the concentration of TNF-α in the culture supernatants evidently increased in dose-dependent manner in HMGB1 groups and prominently decreased by the LALF co-incubated.Conclusion HMGB1 was firstly indicated in this study to be directly inhibited by LALF in late course of endotoxemia.

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What this paper is about

Objective To investigate the effects of limulus anti-lipopolysaccharide factor(LALF) on the late proinflammatory cytokine of high mobility group box 1(HMGB1).Methods The primary mouse macrophages were cultured and stimulated by HMGB1. Macrophage migration was determined by the use of chemotaxis chamber.ELISA quantitated the culture supernatants for TNF-α production.LALF was added to explore the effects of HMGBI and TNF-α production in culture.Results The chemotactic index of macrophages and the concentration of TNF-α in the culture supernatants evidently increased in dose-dependent manner in HMGB1 groups and prominently decreased by the LALF co-incubated.Conclusion HMGB1 was firstly indicated in this study to be directly inhibited by LALF in late course of endotoxemia.

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Available abstract

Objective To investigate the effects of limulus anti-lipopolysaccharide factor(LALF) on the late proinflammatory cytokine of high mobility group box 1(HMGB1).Methods The primary mouse macrophages were cultured and stimulated by HMGB1. Macrophage migration was determined by the use of chemotaxis chamber.ELISA quantitated the culture supernatants for TNF-α production.LALF was added to explore the effects of HMGBI and TNF-α production in culture.Results The chemotactic index of macrophages and the concentration of TNF-α in the culture supernatants evidently increased in dose-dependent manner in HMGB1 groups and prominently decreased by the LALF co-incubated.Conclusion HMGB1 was firstly indicated in this study to be directly inhibited by LALF in late course of endotoxemia.

Key concepts: Lipopolysaccharide, HMGB1, In vitro, Limulus, Proinflammatory cytokine, Macrophage, Tumor necrosis factor alpha, Chemotaxis

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