Screening, Clonging and Analyzing of a Novel Hyperlipidemia Related Gene from Rabbits Liver

Aim To clone a novel rabbit’s gene involved in hyperlipidemia. Methods The hypercholesterolemic rabbits were obtained after feeding atherogenic diet for 150 days. The differentially expressed gene was obtained by suppression subtractive hybridization and rapid amplification of cDNA end. After identified by cDNA microarray and fluorescent semi-quantitative PCR, its tissue distributing was analyzed. Results A novel rabbits gene involved in hypercholesterolemia had been cloned. GenBank Accession No. is AY248719. The full length of cDNA is 1 386 bp and encodes 321 amino acids, which has a 92% identity with that of human GANS1 gene. Conclusions These results will contribute to reveal the molecular mechanism of the hyperlipidemia