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Experimental study on apoptosis of HL-60 cell induced by arsenic trioxide.

Z Wang, Yan Xu, Jian-Rong Liao

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Abstract

OBJECTIVE: To explore the mechanism of arsenic (arsenic trioxide, AS2O3) in treating leukemia. METHODS: The in vitro effect of arsenic trioxide on HL-60 model double-labelled with PI/AnnexinV-FITC was studied ith flow cytometry. Function on telomerase activity was also observed. RESULTS: After the effect of arsenic on HL-60 cell line, PI negative/AnnexinV-FITC positive cells increased, it mainly acts at the G2-M phase that suggests the apoptosis takes place, mild inhibition on telomerase was also found 48 hours later. CONCLUSION: Apoptosis induced by arsenic trioxide was the main mechanism in killing or inhibiting leukemic cells.

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What this paper is about

OBJECTIVE: To explore the mechanism of arsenic (arsenic trioxide, AS2O3) in treating leukemia. METHODS: The in vitro effect of arsenic trioxide on HL-60 model double-labelled with PI/AnnexinV-FITC was studied ith flow cytometry. Function on telomerase activity was also observed. RESULTS: After the effect of arsenic on HL-60 cell line, PI negative/AnnexinV-FITC positive cells increased, it mainly acts at the G2-M phase that suggests the apoptosis takes place, mild inhibition on telomerase was also found 48 hours later. CONCLUSION: Apoptosis induced by arsenic trioxide was the main mechanism in killing or inhibiting leukemic cells.

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Available abstract

OBJECTIVE: To explore the mechanism of arsenic (arsenic trioxide, AS2O3) in treating leukemia. METHODS: The in vitro effect of arsenic trioxide on HL-60 model double-labelled with PI/AnnexinV-FITC was studied ith flow cytometry. Function on telomerase activity was also observed. RESULTS: After the effect of arsenic on HL-60 cell line, PI negative/AnnexinV-FITC positive cells increased, it mainly acts at the G2-M phase that suggests the apoptosis takes place, mild inhibition on telomerase was also found 48 hours later. CONCLUSION: Apoptosis induced by arsenic trioxide was the main mechanism in killing or inhibiting leukemic cells.

Key concepts: Arsenic trioxide, Apoptosis, Arsenic, Chemistry, Telomerase, Flow cytometry, Pi, Cancer research

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