2011•Shipin yanjiu yu kaifaRequires access
Optimization of Conditions of Immobilized Black Soybean Esterase
Meiling Li
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Abstract
Black soybean esterase was immobilized by Cl-type of 717 anion exchange resin coped with acid and alkali solution.Then optimun immobilized conditions were obtained through single factor and orthogonal test.Results show that optimun immobilization conditions were a diluted 50 times solution of crude enzyme,an immobilization temperature of 25 ℃,and an immobilization time of 9 hours.Under these conditions.Meanwhile,the recovery rate of immobilized enzyme was 55.87 %.The inhibition experiments to the Omethoate showed that: the immobilized enzyme had a good linear relationship with the concentration change of Omethoate.The corresponding regression equation was y=17.605x+57.437.