2011•Zhongguo tiaoweipinRequires access

Spectrophotometric determination of lead in food with 2-(2-Bromo-6-Cyano-4-Nitrobenzeneazo) Hydr-oquinone as the chromogenic reagent

Gui-Xia Hao

Abstract

Objective:The aim was to explore the method for the determination of trace lead.Method:With 2-(2-Bromo-6-Cyano-4-Nitrobenzeneazo)Hydroquinone as the chromogenic reagent,the optimum of spectrophotometric determination of lead was studied.Result:In the buffer solution of HAc-NaAc,lead could form orange complex with 2-(2-Bromo-6-Cyano-4-Nitrobenzeneazo)Hydroquinone.The maximum absorption of the complex occurred at 457 nm.The apparent molar absorptivity was 5.13×104 L·mol-1·cm-1,and Beers lawwas obeyed in the range of 0~15 μg/25 mL.,RSD was 2.3%~2.8%.Conclusion:The method has good accuracy and precision.It s a convenient and reliable method for analyzing tea.

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Key concepts: Chromogenic, Reagent, Molar absorptivity, Chemistry, Hydroquinone, Chromatography, Buffer solution, Spectrophotometry