Effect of Different Weaning Diets on Expression Pattern of Hypocreins Receptor 2 in Intestine for Weanling Piglets
Chen Dai-wen
Abstract
Chen Dai-wen
Abstract
To investigate the impact of different weanling diets on expression pattern of Hypocreins Receptor 2(HCRTR2) in intestine for weanlingpiglets,real-time quantitative reverse transcription PCR(RT-qPCR) was carried out to quantify HCRTR2 expression at age of 0,3,7 and 14 days in weanling piglets fed with the diets 30% milk protein substituted the whole protein(treatment 1) and the vegetable protein(treatment 2).RT-qPCR result showed that HCRTR2 expressed in postnatal intestine of piglets during weanling.In the treatment 1,the expression level increased on day 3,7,14 compared with day 0 with 4.30,6.60 and 4.12 times higher respectively and expression on day 7 being highest.In the treatment 2,the expression level increased 1.44 and 4.50 times on day 7,14 while it decreased 0.71 times on day 3 compared with day 0.In conclusion,the different protein diets affect the expression pattern of Hypocreins Receptor 2 in intestine for weanling piglet.
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To investigate the impact of different weanling diets on expression pattern of Hypocreins Receptor 2(HCRTR2) in intestine for weanlingpiglets,real-time quantitative reverse transcription PCR(RT-qPCR) was carried out to quantify HCRTR2 expression at age of 0,3,7 and 14 days in weanling piglets fed with the diets 30% milk protein substituted the whole protein(treatment 1) and the vegetable protein(treatment 2).RT-qPCR result showed that HCRTR2 expressed in postnatal intestine of piglets during weanling.In the treatment 1,the expression level increased on day 3,7,14 compared with day 0 with 4.30,6.60 and 4.12 times higher respectively and expression on day 7 being highest.In the treatment 2,the expression level increased 1.44 and 4.50 times on day 7,14 while it decreased 0.71 times on day 3 compared with day 0.In conclusion,the different protein diets affect the expression pattern of Hypocreins Receptor 2 in intestine for weanling piglet.
Key concepts: Weanling, Weaning, Biology, Receptor, Small intestine, Receptor expression, Protein expression, Real-time polymerase chain reaction