Determination of 1-deoxynojinimycin in different parts of mulberry by HPLC-ELSD

OBJECTIVE To establish HPLC-ELSD method and determine 1-deoxynojinimycin(DNJ) in different parts of different varieties of mulberry.METHODS HPLC analysis was performed on a DIKMA Interesil NHz Columm(250mm×4.6mm,5μm).The mobile phaseconsisted of acetonitrile-methanol-water 170:10:20).The flow rate was 1.0ml·min-1.The temperature was set at 30℃.RESULTS The HPLC-ELSD method could quantitatively determine DNJ in different parts of different varieties of mulberry.The linear range was 1.02-12.24 μg(r=0.9995) and the average recovery was 98.7% with RSD of 1.58%.CONCLUSION The identification methods are reproducible and specific,the quantitative determination method is sensitive and accurate.There is great different of DNJ in difference parts in different varieties of mulberry.The contents of DNJ in M.bombycis Koidz form Lu town(Luzhou city) and Shantai town(Mianyang city)were 8.145,7.624 mg·g-1,respectively.It is middle DNJ in mulberry leaf and minimum in mulberry fruit(the lowest: 0.345 mg·g-1).