Localization and semi-quantitive analysis of podocin in young rats with Adriamycin Neprosis

Objective: To approach the localization of podocin in glomerulus and its possible role in proteinuria occurring in nephrotic syndrome as well as the effect of predisone and benazepril on its density in glomerulus so as to study the possible mechanism of predisone and benazepril in treating NS. Methods:After setting up nephrotic rat models, the rats were randomly divided into 4 groups.Results:1 Podocin was distributed at the base of the podocyte foot processes near the GBM. It was primarily located at the cytoplasmic face of the plasma membrane adjacent to the filtration slits. 2 The staining intensity of podocin decreased prominently in nephrotic samples comparing with normal control group but increased in predisone treated and benazepril treated samples comparing with untreated nephritic samples. Conclusions:Podocin is a major component of the slit diaphram; the concentration of podocin decreased prominently in nephrotic samples; it is possible that predisone and benazepril reduces proteinuria by affecting the intensity of podocin in the glomerulus.