Inhibition effects of shayingtao lipids on the human gastric carcinoma MGC-803 cells

Objective To investigate toxic effect of shayingtao lipids on human gastric carcinoma MGC-803 cells proliferation and its potential mechanism.Methods The living cell were determined by trypan blue method,and the cell apoptosis was detected by flow cytometry.The MGC-803 cells were cultured together with shayingtao lipids of different concentration (shayingtao lipids group),5-FU (5-FU group) and PBS solution (control group) respectively.The inhibition effects of shayingtao lipids and 5-FU on MGC-803 cell proliferation were observed and compared.Results Culturing MGC-803 cells together with 0.5,1.0,3.0 mg / L shayingtao lipids and 10mg/L 5FU for 6h,12h,18h,24h and 48h,the number of living cells decreased along with the increase of shayingtao lipids concentration and prolongation of action time,and the result was similar with 5-FU(P0.05)while had statistical difference compared with control group (P0.01).According to analysis of cell living rate,the proliferation of MGC-803 cells was blocked in G0G1 stage.Culturing MGC-803 cells together with 0.5,1.0,3.0 mg/L shayingtao lipids and 10 mg / L 5-FU for 48h,the apoptosis rates of MGC-803 cell were 13.8%,19.6%,26.2% and 21.1% respectively.Conclusion Shayingtao lipids have significant toxic effect on human gastric carcinoma MGC-803 cells,and the mechanism might be implicated in inhibiting cell division and inducing cell apoptosis.