Involvement of miR-106b in endothelial cells-mediated angiogenesis

Objective To study whether miR-106bis involved in endothelial cells-mediated angiogenesis.Methods miR-106bcultured and transfected with endothelial cells was divided into miR-106bgroup,blank control group and positive control group.RNA was extracted from miR-106b.The transfection efficiency was confirmed by inverse transcription.Endothelial cell tubes formed in matrigel were observed.Apoptosis of transfected miR-106b was assayed by TUNEL assay.Target genes of miR-106bwere detected.Expressions of miR-106band candidate genes were detected by RT-PCR and Western blot,respectively.Results The number of endothelial cell tubes formed in matrigel was significantly less in miR-106bgroup than in blank control group and positive control group.The ratio of formed tubes,signal transduction and mRNA expression level were significantly lower in miR-106bgroup than in positive control group(P 0.05,P0.01).No significant difference was found in apoptosis of transfected miR-106bamong the 3groups(1.19%vs 3.39%,P0.05).Conclusion miR-106binhibits endothelial cells-mediated angiogenesis by down-regulating the signal transduction and STAT3,which is not directly related with VEGFA.