Mutagenicity study of Chiganmaitong Injection

Objective To explore mutagenicity of Chiganmaitong Injection.Methods Three types of tests were performed:Salmonella typhimurium histidine auxotrophic strain reverse mutation assay(Ames test),Chinese hamster lung fibroblasts(CHL) chromosomal aberration test and mouse bone marrow micronucleus test.Results In Ames test,for Chiganmaitong Injection in 312.5-5 000 μg·dish-1dose range,with S9 or not,the number of revertant colonies salmonella typhimurium histidine-deficient TA97,TA98,TA100,TA102 and TA1535 five bacteria had no dose-dependent increase;In chromosome aberration test,in the non-activation conditions or metabolic activation conditions,in 1 200,600,and 300 μg·mL-1 concentration,the cell chromosome aberration had no dose-dependent increased;In micronucleus test,in 1 150,575 and 287.5 mg·kg-1 dose group,there was no statistically significant difference between test sample group and blank control group.Conclusion In the experimental conditions,Ames test,CHL cell chromosome aberration test and mouse bone marrow micronucleus test results were negative,therefore,Chiganmaitong Injection has no mutagenic effect.