Construction and Identification of ER-β SiRNA Expression Vector

Objective:To construct vectors expressing siRNA against human ER-β gene.Methods:Two sequences corresponding to the human ER-β gene were designed.The 2 complementary DNA fragments were synthesized,and then cloned into pSilencer3.1-H1-neo vector for constructing the recombinant plasmid.The recombinant plasmid was transformed into strain DH5α.The plasmid was identified by DNA sequencing.Results:The cloned siRNA construct was inserted into pS ilencer3.1-H1-neo vecto and the inserted sequences were verified by DNA sequencing,thus the human ER-β siRNA expression vector was gained.Conclusion:Recombinant plasmid expressing siRNA of human ER-β gene was constructed successfully.which could provide experimental data for RNA interference of the ER-β gene.