Influence of siRNA-CyclinE on proliferation of BmN cells

【Objective】 This study aimed to study the influence of small interfering RNA on CyclinE gene expression of cell and cell cycle in Bombyx mori ovarian cell lines(BmN).【Method】 siRNA for CyclinE gene fragments at the site of 1 252 and 568 was chemically synthesized before being transfected into BmN cells by lipofectin.Transfection efficiency was observed using confocal fluorescence microscope and then the CyclinE mRNA expression was determined by real-time fluorescent quantitative PCR method.At last,the cell cycle was detected by flow cytometry.【Result】 BmN cells transfection efficiency of siRNA could reach to 80%.48 h after the transfection of 1252-siRNA-CyclinE and 568-siRNA-CyclinE,BmN cells CyclinE mRNA expression were reduced by 68% and 90% respectively.Flow cytometry results in the 2 transfection groups showed that the G0/G1 phase cells ratio increased,while the S and G2/M phase cell ratios decreased.【Conclusion】 The siRNA against CyclinE was imported into BmN cells,and showed effectively inhibition to CyclinE gene expression,and bogged down cell cycle in G1 phase,so as to inhibit cell proliferation.