Effect of IGF-1 on Expression of GH Receptor,IGF-1,IGF-1 Receptor,KAP3.2 and KAP6-1 mRNA in the Skin of Sheep

Thirty-six healthy and similar Xinjiang sheep were selected and divided into six groups at random.Each animal was injected intradermally with 0.5mL IGF-1(10ng/mL)at left pro-scapulae region,while the right pro-scapulae skin was noninjected control.Treatment and control skin tissue of sheep were sampled respectively for 0,3,6,9,12 and 50 days and the skin expression of growth hormone receptor(GHR),insulin-like growth factor 1(IGF-1),insulin-like growth factor receptor 1(IGF-1R),KAP3.2 and KAP6-1 mRNA were measured by RT-PCR.The results indicated that IGF-1 could degrade GHR gene expression,have no effect of IGF-1 and IGF-1R gene expression,and increase significantly KAP3.2 and KAP6-1 gene expression.Taken together these findings that the improvement of KAP3.2 and KAP6-1 gene expression may correlate with other pathway beyond the insulin-like growth factor axis.