Establishment of Transgenic Cell Lines of human 160 gene and Preliminary Study of Its Biological Characteristics

Objective To clone human CD160 gene and construct recombinant plasmid vector carrying the target gene which could be expressed stably in cell line L929.Methods The CD160 gene was amplified by RT-PCR from human peripheral blood mononuclear cells.Besides,transmembrane region gene was amplified by PCR using the template from the whole cDNA of VSIG4 gene.And it was inserted into eukaryotic expressing vector pIRES2-EGFP.The recombinant vector was then transfected into L929 cells.After being selected by G418,transgenic cell line expressing stably human CD160 was established.Results The gene of CD160 was successfully cloned,and its recominant vector pIRES2-EGFP/CD160TMV was constructed.The transfectant stably expressing human CD160 protein on the cells membrane was established successfully.This transfectant could bind to another transgenic cell line L929/HVEM through co-culture.Conclusion The recombinant vector and the transgenic cell lines stably expressing human CD160 molecule on the cells surface has been obtained and can be used for further biological function research.