Optimization of Extraction Technology and Investigation of in vitro Antopxidation for Total Flavonoids from Lysimachia paridiformis

Objective:To optimize extraction technology of total flavonoids from Lysimachia paridiformis,and investigate its in vitro antopxidation.Method:With yield of total flavonoids as index,single factor and orthogonal test were used to optimized extraction technology of total flavonoids from L.paridiformis with solid-liquid ratio,the concentration of ethanol,reflux time and temperature as factors;Scavenging action and reducing power of total flavonoids on hydroxyl radical and superoxide anion free radical were determined by UV spectrophotometry.Result:Optimum extraction technology of total flavonoids from L.paridiformis was:ethanol concentration 65%,ratio of solid-liquid 1∶25,reflux temperature 65 ℃,extraction time 4 h,yield of total flavonoids of 1.69%;Total flavonoids from L.paridiformis had good reducing power and ability to inhibit free radical,and reducing power and scavenging hydroxyl radical activity increased with increasing the concentration of total flavonoids.Conclusion:total flavonoids from L.paridiformis had significant antopxidation.