An investigation on high-risk HPV infections and subtype distribution in women from Shaoxing area

Objective To explore the rate of high-risk Human papillomavirus( HPV) infections and genotypes distribution in women with cervical lesions from Shaoxing area. Methods 4273 patients in our hospital from Jan. 2010 to Sep. 2013 were enrolled in this study. The thinprep cytology tests were performed in all patients. The subgroup of HPV was determined by PCR-based reverse blot hybridization( PCR-RDB),HPV and high-risk HPV( HR-HPV) infection rate and the distribution of genotypes were compared among different groups by the statistical analysis. Results The positive rate of HPV was 21. 8%( 932 /4273). The infection ratio of HPV were 15. 5%( 601 /3888),83. 7%( 236 /282) and 92. 2%( 95 /103) in woman with NILM,ASC and SIL,respectively. The constituent ratio of HR-HPV were 83. 0%( 601 /724),94. 8%( 274 /289) and 96. 7%( 118 /122) in NILM,ASC and SIL,respectively; The five most common prevalent HRHPV were HPV-16,HPV-52,HPV-58,HPV-33 and HPV-68 in NILM,HPV-16,HPV-58,HPV-52,HPV-18 and HPV-33 in ASC patients,and HPV-16,HPV-58,HPV-33,HPV-52 and HPV-31 in SIL patients. HPV multiple infection rate of in HPV-positive women with NILM( 17. 6%,106 /601) was no significant difference that of HPV-positive women with ASC( 16. 9%,40 /236) and with SIL( 18. 9%,18 /95),χ2≤0. 2,P 0. 05. Conclusion The infection ratio of HPV and constituent ratio of HR-HPV were closely related to the degree of cervical diseases. Multiple infection of HPV cannot aggravate the cervical lesions. HPV-16,HPV-58 and HPV-52 were the most common prevalent HR-HPV in HPV-positive women with cervical lesions,which is different with European and American countries.