2008•Yunnan Daxue xuebao. Shehui kexue banRequires access

Application of solid phase extraction spectrophotometry with SBDR in analysis of leadin tobacco

Yi Li

Abstract

The color reaction of plumbeous with p-sulfobenzylidenerhodanine(SBDR) was studied in a pH=4.0 HAc-NaAc buffer solution and Tween-60 medium.This complex can be extracted by Waters Sep-Park-C18 cartridge and eluted by ethanol.The molar absorptivity is 1.12×105 L·mol-1·cm-1 at 560 nm.Beer's law is obeyed in the range of 0—6 μg/50 mL.This method can be applied to the determination of plumbeous in tobacco with satisfactory results.

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Key concepts: Molar absorptivity, Spectrophotometry, Solid phase extraction, Extraction (chemistry), Elution, Chromatography, Chemistry, Buffer solution