Experimental Study on Borrelidin's Function to the Bid/Bcl-xl and Caspase-8 in Endothelial Cells of Mice Spleen Sinus

Objective: To evaluate the ratio of Bid/Bcl-xl and change of Caspase-8 before and after Borrelidin's effect on the spleen sinus endothelial cells of mice.Methods: 60 SPF male mice aging nine weeks are divided into four groups: NC group,NS group,BO3d group and BO7d group.Mice in NC group are normally raised without any treatment.Mice in NS group are injected saline.Mice in BO3d and BO7d group are injected 0.5ml Borrelidin.After 3 days mice in NC,NS and BO3d groups are executed and mice in BO7d group are executed after 7 days.The apoptotic cells are marked by TUNEL to get AI.Immunohistochemical method is used to detect the expression of apoptosis related proteins Bid,Bcl-xl and Caspase-8.Image analysis technique is used to compare integral light density,calculate the ratio of Bid/Bcl-xl and analyze the relationship between them.Results: The apoptosis in BO3d and BO7d group are widely present in the spleen sinus endothelial cells.The expression of Bcl-xl is present in NC and NS groups with certain amount but no significant difference.Expression of Bcl-xl is slightly declined as drug functions continue.There is significant difference between BO7d and NC groups.The expression of Caspase-8 and Bid is consistent with the positive expression of Bid/Bcl-xl and apoptosis.The expression of Bcl-xl is negatively correlated with AI.The expression of Caspase-8 and Bid and the ratio of Bid/Bcl-xl is positively correlated with AI.Conclusion: Borrelidin is capable of activating the Bid protein through starting Caspase-8,which in a certain extent antagonizes inhibiting apoptosis roles of Bcl-xl,promotes the Bid/Bcl-xl ratio,and induces overall apoptosis trends of spleen sinus endothelial cell of mice.