Cloning of Major Outer Membrane Protein Antigen Gene of Legionella pneumophila and Detection of Its Expression in Prokaryotic Cell

Objective To clone the major outer membrane antigen gene omp M of Legionella pneumophila, to construct recombinant plasmid pLPM and to dete ct its expre ssion in prokaryotic cell strain JM109.Methods The ompM gene fr o m Legionella pneumophila was amplified with PCR. The PCR product was cloned into pUC18 vector a nd ompM expressed in JM109. After that, the expression of JM109 of ompM gene was det ected with SDS-PAGE. Results The ompM gene of 773bp was amplif ied, the recombin ant plasmid pLPM constructed and protein of 25 kDa expressed in JM109.C onclusion The ompM gene was amplified, the recombinant plasmid pLPM cons tructed and its expression detected in prokaryotic cell successfully.