Study on protective effect and its mechanism of pretreatment of Caspase-3 gene inhibiter on hypoxic-ischemic brain damage in the neonatal rats

Objective To study the effect of hypoxia ischemia brain damage(HIBD)of neonatal rats with cerebral cortex apoptosis by gene inhibiter so as to explore the possible mechanism. Methods Model of HIBD was built in neonatal rats,60Wistar rats were randomly divided in:control group;HIBD group;obstruct group(HIBD+z-VAD-fmk).Adopting HE dyeing,three animal apoptosis index(AI)in brain and Caspase-3mRNA were detected by TUNEL and RT-PCR. Results Express of Caspase-3mRNA nanomolar in control group was significant heighten in HIBD group(P0.01).There was significantly lower express between obstruct group and HIBD group(P0.05).AI value changed with Caspase-3mRNA.They had significance positive correlation(r=0.628,P0.05). Conclusion Express heigten of Caspase-3mRNA leads to histiocytic apoptosis in HIBD group,and lower express of Caspase-3mRNA by z-VAD-fmk,could protect brain of neonatal rats.