ESTABLISHMENT OF HIGH-THROUGHPUT TECHNICALPLATFORM FOR TUMOR ANGIOGENESIS RESEARCH

ve To establish in vitro in vivo high-throughput technical platform for tumor angiogenesis research.Methods VEGF and bFGF as angiogenic factors, thalidomide asan angiogenesis inhibitor, various properties of vascular endothelialcells (RF/6A and SVEC) were detected in vitro with MTT assay,wound migration assay, invasion assay and tube formation assay.The effects of angiogenic factors and inhibitor on angiogenesis weredetermined in vivo in clean grade chicken embryos and C57BL/6mice as experimental animals.Results Angiogenesis was promotedand accelerated by VEGF and bFGF in vitro and in vivo.In vitroresults showed that the viability,migration,invasion and tubeformation were significantly stimulated and enhanced by VEGF orbFGF protein(P 0.0l). Mouse matrigel plug assay and CAMassay also indicated that angiogenesisi was stimulated andaccelerated significantly by VEGF and bFGF in vivo. Compared tonegative control, animals treated with VECF and bFGF had muchmore new blood vessels with high density, larger diameter and muchintegrity in CAM and matrigel plug. Angiogenesis was markedlyinhibited by thalidomide in vitro and in vivo. Conclusion Thisplatform is high-throughput, economic, simple and useful inangiogenesis research.