2011•Shanxi Yike Daxue xuebaoRequires access
Detection of arsenic contents in blood,urine and organs of rats exposed to arsenic through drinking water
Qiu-ling Pei
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Abstract
Objective To investigate the contents of arsenic in blood,urine,liver,kidney,lung,heart,spleen,and brain of rats after exposure to arsenic.Methods Thirty-two healthy Wistar rats were randomly divided into four groups.The rats were exposed to arsenic through drinking water at doses of 0(control group),50,150,450 μg/ L for four weeks,respectively.The water consumption per day per rat was about 37 ml.Rats were sacrificed after four weeks.The weight of liver,kidney,lung,heart,spleen and brain were recorded,organ coefficients were calculated and contents of arsenic in blood,urine and organs were detected.Results Compared with control group,the body weight in low dose group was higher at the end of 2 weeks(P0.05),and the body weight in middle and high dose groups was lower at the end of 4 weeks(P0.05).The liver organ coefficient in low and middle dose groups were significantly higher than that in control group(P0.05),but there was no significant difference in organ coefficients of kidney,lung,heart,spleen and brain among 4 groups.The levels of arsenic in blood,urine,spleen and kidney were significantly higher in three arsenic groups than in control group.Arsenic levels in heart,liver,lung and brain were higher in middle and high-dose groups than that in control group(P0.05).Arsenic contents in blood,urine,liver,kidney,lung,heart,spleen and brain increased in a dose-dependant manner.The arsenic level was significantly different among different organs in the same group,and it was the highest in spleen and the lowest in brain(P0.05).The arsenic contents in blood and urine were significantly correlated with the contents of arsenic in liver,kidney,lung,heart,spleen,brain(P0.05).Conclusion Arsenic can accumulate in certain important organs of rats after exposure to arsenic,but the distribution of arsenic in various organs is different.Both blood arsenic and urine arsenic can reflect the burden of body in rats to some extent.