Experiment study of Jisuikang on treatment of rat after spinal cord injury

Objective:To observe the effect of Jisuikang on treatment of rats after empirical spinal cord injury,and understand whether has dose-dependent effect or not,and perform positive control with prednisone.Methods:Seventy-two SD rats were separated randomly into six groups(n=12).The rats in the Jisuikang high-dose group,middle-dose group and low-dose group were given Jisuikang 25、12.5、6.25 g/kg for 14 days.The rats in the prednisone group were given 0.06 g/kg prednisone for 7 days.The rats in normal and model group were given distilled water of the same volume.After one hour of last administration,collecting the blood in rats' fossa orbitalis of every group,testing the content of IL-10、TNF-α and NO、SOD、NOS、MDA in the blood;testing whole blood viscosity;testing the content of NO、SOD、NOS、MDA in the injuried spinal cord tissue.Spinal cord histopathologic examination was done ater determination.Results:After modeled,the content of TNF-α in the blood of rats was raised compared with normal group,and this in Jisuikang high-dose group(P0.01),middle-dose group(P0.05)and low-dose group(P0.01)was decreased compared with model group.After modeled the content of IL-10 in the blood of rats was obviously decreased compared with normal group,and this in Jisuikang high-dose group,middle-dose group,low-dose group and prednisone group was significant difference as compared with model group(P0.01).After modeled,the rats' whole blood viscosity was significant difference as compared with normal group.And this in prednisone group had no difference as compared with model group.But this in Jisuikang high-dose group and middle-dose group was lower than this in model group.After modeled,the content of SOD in the blood was obviously decreased but MDA,NO and NOS raised.The content of SOD in Jisuikang high-dose group and middle-dose group was higher than this in model group,but the MDA content in the two groups was lower than this in model group.The content of NOS in Jisuikang high-dose group,middle-dose group and low-dose group was lower than this in model group.The content of NO in Jisuikang high-dose group and middle-dose group was lower than this in model group.Compared with model group,the MDA content in prednisone group was lower,but the SOD content had no change.After modeled,the content of SOD in the injuried spinal cord tissue was obviously decreased but MDA,NO and NOS raised.The content of SOD in Jisuikang high-dose group was higher than model group.The content of MDA and NOS in Jisuikang high-dose group and middle-dose group were both lower than these in model group.The content of NO in Jisuikang high-dose group,middle-dose group and low-dose group was lower than this in model group.Compared with model group,the MDA content in the injuried spinal cord tissue in prednisone group was lower,but the SOD content had no change.Results of pathological examination:The pathological change of spinal cord tissue in Jisuikang every group was lighter,especially in Jisuikang high-dose group.The pathological change in prednisone group was similar as this in Jisuikang high-dose group and middle-dose group.Conclusion:Jisuikang can inhibit NOS expressing and degrade the content of NO.Jisuikang can degrade the content of MDA and raise the content of SOD.Jisuikang can improve the index of hemorheology.Jisuikang can degrade the content of TNF-α.Jisuikang can raise the content of IL-10.Jisuikang can prevent secondum change of spinal cord injury,promote recovery of spinal cord tissue and regeneration of nerve tissue.The therapeutic effect of Jisuikang high-dose group was more significant than Jisuikang middle-dose group.