Tao Yong-ping
Abstract
Total RNA was prepared from liver of Tibetan sheep,and RT-PCR method was employed to obtain a 589 bp-lenghth cDNA of Tibetan sheep IGF-I gene,encoding peptide with 70 amino acids.The homology of nucleotide sequence of IGF-I between Tibetan sheep and other sheep breeding was 99.78%,with only one nucleotide difference.
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Total RNA was prepared from liver of Tibetan sheep,and RT-PCR method was employed to obtain a 589 bp-lenghth cDNA of Tibetan sheep IGF-I gene,encoding peptide with 70 amino acids.The homology of nucleotide sequence of IGF-I between Tibetan sheep and other sheep breeding was 99.78%,with only one nucleotide difference.
Key concepts: Complementary DNA, Cloning (programming), Biology, Gene, Homology (biology), Nucleic acid sequence, Nucleotide, Amino acid