ScholarLens Back to search
2004•Zhongguo shuxue zazhiRequires access

In vitro study of bone marrow-endothelial progenitor cells

Wang Wei

Open publisher page 0 citations

Abstract

Objective To explore the expansion and differentiation of bone marrow endothelial progenitor cells in vitro. Methods Rabbit bone marrow mononuclear cells were isolated by gradient density centrifugation.Attached cells were cultured and in endothelial cell growth supplement(ECGS) from bovine pituitary.Cell multiplication was observed,and their biological characteristics were detected. Results The number of attached cells increased 5～8 fold with each expanded.Cobblestone like structure was observed when the cells lined in single layer.Expanded cells expressed von Willebrand factor(vWF) and could ingest Dil acLDL. ConclusionEndothelial progenitor cells,which exist within adult bone marrow,have the potential to be expanded and differentiated when stimulated by ECGS in vitro.

About this research paper

What this paper is about

Objective To explore the expansion and differentiation of bone marrow endothelial progenitor cells in vitro. Methods Rabbit bone marrow mononuclear cells were isolated by gradient density centrifugation.Attached cells were cultured and in endothelial cell growth supplement(ECGS) from bovine pituitary.Cell multiplication was observed,and their biological characteristics were detected. Results The number of attached cells increased 5～8 fold with each expanded.Cobblestone like structure was observed when the cells lined in single layer.Expanded cells expressed von Willebrand factor(vWF) and could ingest Dil acLDL. ConclusionEndothelial progenitor cells,which exist within adult bone marrow,have the potential to be expanded and differentiated when stimulated by ECGS in vitro.

Why it matters

A significance statement is not available in the OpenAlex record.

Key contribution

A contribution statement is not available in the OpenAlex record.

Method / approach

Method details are not available in the OpenAlex metadata.

Main findings

Findings are not separately available in the OpenAlex metadata.

Limitations

Limitations are not available in the OpenAlex metadata.

Applications

Application details are not available in the OpenAlex metadata.

Available abstract

Objective To explore the expansion and differentiation of bone marrow endothelial progenitor cells in vitro. Methods Rabbit bone marrow mononuclear cells were isolated by gradient density centrifugation.Attached cells were cultured and in endothelial cell growth supplement(ECGS) from bovine pituitary.Cell multiplication was observed,and their biological characteristics were detected. Results The number of attached cells increased 5～8 fold with each expanded.Cobblestone like structure was observed when the cells lined in single layer.Expanded cells expressed von Willebrand factor(vWF) and could ingest Dil acLDL. ConclusionEndothelial progenitor cells,which exist within adult bone marrow,have the potential to be expanded and differentiated when stimulated by ECGS in vitro.

Key concepts: Bone marrow, Progenitor cell, In vitro, Peripheral blood mononuclear cell, Endothelial stem cell, Cell biology, Immunology, Biology

Related papers

Related paper 1Related paper 2Related paper 3
Back to paper searchBrowse research topicsOriginal source
In vitro study of bone marrow-endothelial progenitor cells — Research Paper | ScholarLens