Effect of Ad-14-3-3σ on microRNA expression in different radioresistant nasopharyngeal carcinoma cells

AIM:To discuss the effect of Ad-14-3-3σ to microRNA(miRNA)in different radioresistant nasopharyngeal carcinoma(NPC)cells,CNE-1 and CNE-2,and study the relationship between the discrepancy of miRNA and radiosensitivity of NPC.METHODS:Ad-14-3-3σ was transfected to CNE-1 and CNE-2 cells,and then miRNAs were detected by Paraflo microfluidic microRNA chip.Hybridization images were collected using a laser scanner and the signals were normalized using a LOWESS filter.The effect of Ad-14-3-3σ to miRNAs and the relationship between the discrepancy of miRNA and radiosensitivity of NPC were studied according to Targetscan3.1 database(http://www.targetsan.org)after analyzing data.RESULTS:After treated by Ad-14-3-3σ,comparing to CNE-2 cells,there are 37 miRNAs changed remarkably,including 17 over-expression microRNAs and 20 under-expression microRNAs in CNE-1 cells.6 miRNAs that one detective value was more than 1 000 and 3 folds than the other were hsa-miR-152,hsa-miR-205,hsa-miR-203,hsa-miR-7,hsa-miR-636 and hsa-miR-100.CONCLUSION:Ad-14-3-3σ can change the expression of miRNAs in different radioresistant nasopharyngeal carcinoma,and some miRNAs have relevance to carcinoma and radiosensitivity.