2012•China Journal of Chinese Materia MedicaRequires access
Water-soluble chemical constituents from Elaeagnus pungens leaves
Mengshun Li
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Abstract
OBJECTIVE: To study water-soluble chemical constituents from the leaves of Elaeagnus pungens. METHOD: Chemical constituents of E. pungens leaves were separated by a combination of macroporous resin column chromatography, reverse phase silica gel column chromatography, Sephadex LH-20 column chromatography and semi-preparative HPLC. Their structures were identified on the basis of physicochemical properties using the spectral method. RESULT: The two compounds were separated from E. pungens leaves and identified as kaempferol 3-O-P-D-glucopyranosyl- (1-->3)-alpha-L-rhamn-opyranosyl-(1-->6) -/3-D-galactopyranoside (1), kaempferol 3-O-P-D-glucopyranosyl-(1-->3)-alpha-L-rhamnopyranosyl-(1-->6)-beta-D-glucopyranoside-7-O-beta-D-glucopyranoside (2). CONCLUSION: Compound 2 separated from E. pungens leaves was a new compound.