Analysis of the Promoter of Human Spermatogenesis-Related Novel Gene ZNF313

To analyze the promoter of human spermatogenesis-related novel gene ZNF313, the promoter sequence of ZNF313 gene was amplified by PCR and luciferase expression vectors containing different promoter fragments of ZNF313 were constructed. Using pRL-TK plasmid as control, the expression vectors were instantly transfected into HEK293T cell line and the relative activity of luciferase was measured 48 h later. The result indicated that four luciferase expression vectors, pGL3-215 (-215 bp～+38 bp), pGL3-160 (-160 bp～+38 bp), pGL3-133 (-133 bp～+128 bp) and pGL3-8 (-8 bp～+128 bp) were constructed. The relative activity of luciferase of pGL3-215 was the highest, the activities of pGL3-160 and pGL3-133 were much the same, and 75% of the highest activity and the activity of pGL3-8 significantly declined nearly to zero. These indicated that the region from -133 bp to -8 bp included the essential promoter sequence for human ZNF313 gene transcription. The analysis of bioinformatics showed that two SP1 elements, one AP-2 element and one T-Ag element were also necessary for the transcription of human ZNF313 gene. Fig 4, Ref 19